INPP5F Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA sample test of MP02978-2

  The mean INPP5F concentration was determined to be 17.30 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 11.30 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 6.40 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 7.80 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load, and 4.80 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02978-2

  Sandwich ELISA standard curve of MP02978-2, Human INPP5F Recombinant Matched Antibody Pair - PBS only. 87311-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag39933. 87311-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

INPP5F Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 87311-4-PBS

Clone No.252661A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

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Product Information

87311-4-PBS targets INPP5F as part of a matched antibody pair:

MP02978-2: 87311-4-PBS capture and 87311-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag39933

  Product name: Recombinant human INPP5F protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 788-1132 aa of BC111493

  Sequence: VKQTKSNVNIGNLRKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENTGVMDKVQAESDGDMSSDNDSYHSDEFLTNSKSDEDRQLANSLESVGPIDYVLPSCGIIASAPRLGSRSQSLSSTDSSVHAPSEITVAHGSGLGKGQESPLKKSPSAGDVHILTGFAKPMDIYCHRFVQDAQNKVTHLSETRSVSQQASQERNQMTNQVSDETQSESTEQTPSRPSQLDVSLSATGPQFLSVEPAHSVASQKTPTSASSMLELETGLHVTPSPSESSSSRAVSPFAKIRSSMVQVASITQAGLTHGINFAVSKVQKSPPEPEIINQVQQNELKKMFIQCQTRIIQI

• Full Name: inositol polyphosphate-5-phosphatase F
• Calculated Molecular Weight: 1132 aa, 128 kDa
• GenBank Accession Number: BC111493
• Gene Symbol: INPP5F
• Gene ID (NCBI): 22876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2H2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.