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WB analysis using 87311-1-RR (same clone as 87311-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87311-1-RR (INPP5F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87311-1-PBS in a different storage buffer formulation.
WB analysis using 87311-1-RR (same clone as 87311-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87311-1-RR (INPP5F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87311-1-PBS in a different storage buffer formulation.
WB analysis using 87311-1-RR (same clone as 87311-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87311-1-RR (INPP5F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87311-1-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02978-2
The mean INPP5F concentration was determined to be 17.30 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 11.30 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 6.40 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 7.80 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load, and 4.80 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.
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The mean INPP5F concentration was determined to be 17.30 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 11.30 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 6.40 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 7.80 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load, and 4.80 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.
Sandwich ELISA standard curve of MP02978-2
Sandwich ELISA standard curve of MP02978-2, Human INPP5F Recombinant Matched Antibody Pair - PBS only. 87311-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag39933. 87311-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
Affinity of 87311-1-RR
Biolayer interferometry (BLl) kinetic assays of 87311-1-RR against Human INPP5F were performed. The affinity constant is 0.552 nM.

INPP5F Recombinant monoclonal antibody, PBS Only (Detector)

Name: INPP5F Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 87311-1-PBS

INPP5F Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 87311-1-PBS

Clone No.252661F2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

EC:3.1.3.25, hSAC2, Inositol polyphosphate 5-phosphatase F, KIAA0966, MSTP007

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87311-1-PBS targets INPP5F as part of a matched antibody pair:

MP02978-2: 87311-4-PBS capture and 87311-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag39933 Product name: Recombinant human INPP5F protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 788-1132 aa of BC111493 Sequence: VKQTKSNVNIGNLRKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENTGVMDKVQAESDGDMSSDNDSYHSDEFLTNSKSDEDRQLANSLESVGPIDYVLPSCGIIASAPRLGSRSQSLSSTDSSVHAPSEITVAHGSGLGKGQESPLKKSPSAGDVHILTGFAKPMDIYCHRFVQDAQNKVTHLSETRSVSQQASQERNQMTNQVSDETQSESTEQTPSRPSQLDVSLSATGPQFLSVEPAHSVASQKTPTSASSMLELETGLHVTPSPSESSSSRAVSPFAKIRSSMVQVASITQAGLTHGINFAVSKVQKSPPEPEIINQVQQNELKKMFIQCQTRIIQI Predict reactive species
Full Name	inositol polyphosphate-5-phosphatase F
Calculated Molecular Weight	1132 aa, 128 kDa
Observed Molecular Weight	128-140 kDa
GenBank Accession Number	BC111493
Gene Symbol	INPP5F
Gene ID (NCBI)	22876
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9Y2H2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.