INPPL1 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• INPPL1 Recombinant monoclonal antibody (87161-1-RR)

• 

  WB analysis using 87161-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87161-1-RR (INPPL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 87161-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 87161-1-RR (INPPL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

INPPL1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87161-1-RR

Clone No.252293A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.3.86, Inositol polyphosphate phosphatase-like protein 1, INPPL 1, INPPL-1, Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2

---

Tested Applications

• Positive WB detected in: K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87161-1-RR targets INPPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inositol polyphosphate phosphatase-like 1
• Calculated Molecular Weight: 1258 aa, 139 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC140853
• Gene Symbol: INPPL1
• Gene ID (NCBI): 3636
• RRID: AB_3745380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15357
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone.