INSC Polyclonal antibody

INSC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20973-1-AP

Synonyms

INSC, Protein inscuteable homolog

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Validation Data Gallery

• 

  WB analysis of mouse liver using 20973-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20973-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20973-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20973-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20973-1-AP

  Immunofluorescent analysis of HeLa cells using 20973-1-AP (INSC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• ChIP: See 1 publications below

Product Information

20973-1-AP targets INSC in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inscuteable homolog (Drosophila)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_001031853
• Gene Symbol: INSC
• Gene ID (NCBI): 387755
• RRID: AB_10951111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INSC may function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. It is involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. INSC may play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. The antibody can recognize isoform1, 2,4,5 of INSC.

Publications

Species	Application	Title
	ChIP	Cell Rep; The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus.; Authors - Bhavana Gupta