INTS12 Polyclonal antibody

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• INTS12 Polyclonal antibody (16455-1-AP)

INTS12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16455-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Int12, PHF22, SBBI22

• 

  WB analysis of HepG2 using 16455-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16455-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 16455-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16455-1-AP (INTS12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16455-1-AP targets INTS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9403

  Product name: Recombinant human INTS12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 106-462 aa of BC014442

  Sequence: SDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCARCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSASVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKTTSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK

• Full Name: integrator complex subunit 12
• Calculated Molecular Weight: 462 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014442
• Gene Symbol: INTS12
• Gene ID (NCBI): 57117
• RRID: AB_2127263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS12 is a subunit of the Integrator complex that is thought to act as a reader to link the complex to chromatin.

Publications

What published studies show

The INTS12 Polyclonal antibody (Cat# 16455-1-AP) has 4 total citations. These publications appear in Nature Communications, Molecular Cell, Cell Reports, and PLoS Genetics. The research fields span developmental ciliopathy and Integrator complex assembly, RNA polymerase II transcription regulation, neurodevelopmental disorders linked to mitosis defects, and transcriptome integrity in brain development. All cited applications were Western blot (WB) in human samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Mol Cell; Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint; Authors - Daniel Blears
human	WB	Cell Rep; A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder; Authors - Hanzhe Kuang
human	WB	PLoS Genet; Human mutations in integrator complex subunits link transcriptome integrity to brain development.; Authors - Renske Oegema