INTS12 Polyclonal antibody

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  WB analysis of HepG2 using 16455-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16455-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 16455-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16455-1-AP (INTS12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

INTS12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16455-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Int12, PHF22, SBBI22

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

16455-1-AP targets INTS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9403

  Product name: Recombinant human INTS12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 106-462 aa of BC014442

  Sequence: SDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCARCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSASVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKTTSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK

• Full Name: integrator complex subunit 12
• Calculated Molecular Weight: 462 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014442
• Gene Symbol: INTS12
• Gene ID (NCBI): 57117
• RRID: AB_2127263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS12 is a subunit of the Integrator complex that is thought to act as a reader to link the complex to chromatin.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint; Authors - Daniel Blears
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	PLoS Genet; Human mutations in integrator complex subunits link transcriptome integrity to brain development.; Authors - Renske Oegema
human	WB	Cell Rep; A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder; Authors - Hanzhe Kuang