INTS4 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HepG2 using 83590-5-RR (same clone as 83590-5-PBS)

  WB result of INTS4 antibody (83590-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells. This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83590-5-RR (same clone as 83590-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83590-5-RR (same clone as 83590-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83590-5-RR (same clone as 83590-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug INTS4 Recombinant antibody (83590-5-RR, Clone:240579H4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83590-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00580-1

  Cytometric bead array standard curve of MP00580-1, INTS4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83590-5-PBS. Detection antibody: 83590-2-PBS. Standard: Ag9116. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83590-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83590-5-RR against Human INTS4 were performed. The affinity constant is 0.146 nM.

• Featured Product
• KD/KO Validated

INTS4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83590-5-PBS

Clone No.240579H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

240579H4, Int4, MST093, MSTP093

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Product Information

83590-5-PBS targets INTS4 as part of a matched antibody pair:

MP00580-1: 83590-5-PBS capture and 83590-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INTS4 fusion protein Ag9116
• Full Name: integrator complex subunit 4
• Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC009995
• Gene Symbol: INTS4
• Gene ID (NCBI): 92105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96HW7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).