INTS4 Recombinant antibody

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  WB analysis of HepG2 using 83590-5-RR

  WB result of INTS4 antibody (83590-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells.

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  IF Staining of HeLa using 83590-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 83590-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-5-RR, Clone: 240579H4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 83590-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug INTS4 Recombinant antibody (83590-5-RR, Clone:240579H4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83590-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83590-5-RR against Human INTS4 were performed. The affinity constant is 0.146 nM.

• Featured Product
• KD/KO Validated

INTS4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83590-5-RR

Clone No.240579H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240579H4, Int4, MST093, MSTP093

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IF/ICC detected in: U2OS cells, HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83590-5-RR targets INTS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INTS4 fusion protein Ag9116
• Full Name: integrator complex subunit 4
• Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC009995
• Gene Symbol: INTS4
• Gene ID (NCBI): 92105
• RRID: AB_3671204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96HW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).