INTS5 Polyclonal antibody

INTS5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14069-1-AP

Synonyms

INTS 5, Int5

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Validation Data Gallery

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  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14069-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14069-1-AP

  IP result of anti-INTS5 (IP:14069-1-AP, 4ug; Detection:14069-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14069-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14069-1-AP (INTS5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, NIH/3T3 cells, SKOV-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

14069-1-AP targets INTS5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS5 fusion protein Ag5129
• Full Name: integrator complex subunit 5
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC060841
• Gene Symbol: INTS5
• Gene ID (NCBI): 80789
• RRID: AB_2296187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Cell Rep; A combinatorial approach to uncover an additional Integrator subunit; Authors - Sarah R Offley
human	WB	Cell Rep; RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex.; Authors - Xinxin Wang
human	WB	Mol Cell; INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II; Authors - Shibin Hu
human	WB	Mol Cell; Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function; Authors - Min-Han Lin