INTS5 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• INTS5 Polyclonal antibody (14069-1-AP)

• Featured Product
• KD/KO Validated

INTS5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14069-1-AP Copied!

Publications (9)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA, CUT&Tag

Synonyms

Int5, INTS 5

• 

  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14069-1-AP

  WB result of INTS5 antibody (14069-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS5 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14069-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 14069-1-AP

  IP result of anti-INTS5 (IP:14069-1-AP, 4ug; Detection:14069-1-AP 1:1000) with HeLa cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 14069-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14069-1-AP (INTS5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for INTS5 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, NIH/3T3 cells, SKOV-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14069-1-AP targets INTS5 in WB, IHC, IF/ICC, IP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5129

  Product name: Recombinant human INTS5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-322 aa of BC060841

  Sequence: MSALCDPPGAPGPPGPAPATHGPAPLSAQELSQEIKAFLTGVDPILGHQLSAREHARCGLLLLRSLPPARAAVLDHLRGVFDESVRAHLAALDETPVAGPPHLRPPPPSHVPAGGPGLEDVVQEVQQVLSEFIRANPKAWAPVISAWSIDLMGQLSSTYSGQHQRVPHATGALNELLQLWMGCRATRTLMDIYVQCLSALIGSCPDACVDALLDTSVQHSPHFDWVVAHIGSSFPGTIISRVLSCGLKDFCVHGGAGGGAGSSGGSSSQTPSTDPFPGSPAIPAEKRVPKIASVVGILGHLASRHGDSIRRELLRMFHDSLA

• Full Name: integrator complex subunit 5
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC060841
• Gene Symbol: INTS5
• Gene ID (NCBI): 80789
• RRID: AB_2296187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P9B9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Publications

What published studies show

The INTS5 Polyclonal antibody (Cat# 14069-1-AP) has 9 citations published in Science, Nature Communications, Molecular Cell, and Cell Reports. The cited studies focus on the Integrator complex and its roles in RNA polymerase II transcription, snRNA processing, ciliopathy, nucleosome remodeling at cis-regulatory elements, transcription initiation checkpoints, and coupling of transcription elongation with RNA processing. Applications used include WB, IP, and CUT&Tag in human and mouse samples.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB,IP	Nat Commun; DSS1 is required for proper Integrator-PP2A function; Authors - Congling Xu
human	WB	Mol Cell; INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II; Authors - Shibin Hu
human	WB	Mol Cell; Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function; Authors - Min-Han Lin
human	WB	Cell Rep; A combinatorial approach to uncover an additional Integrator subunit; Authors - Sarah R Offley