INTS8 Polyclonal antibody

INTS8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18802-1-AP

Synonyms

C8orf52, INT8, integrator complex subunit 8, INTS8, Protein kaonashi 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 18802-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18802-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18802-1-AP

  IP result of anti-INTS8 (IP:18802-1-AP, 4ug; Detection:18802-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IF Staining of HeLa using 18802-1-AP

  Immunofluorescent analysis of Hela cells, using INTS8 antibody 18802-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

18802-1-AP targets INTS8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrator complex subunit 8
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 90-115 kDa
• GenBank Accession Number: BC136754
• Gene Symbol: INTS8
• Gene ID (NCBI): 55656
• RRID: AB_10597250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS8, also named as C8orf52, is a Component of the Integrator complex which is a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. INTS8 is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	J Exp Clin Cancer Res; Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma.; Authors - Fuqiang Yin
human	IHC	Cancer Manag Res; INTS8 accelerates the epithelial-to-mesenchymal transition in hepatocellular carcinoma by upregulating the TGF-β signaling pathway.; Authors - Hui Tong
human	WB	Cell Rep; A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder; Authors - Hanzhe Kuang
human	WB	Elife; Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9; Authors - Joshua D Eaton