IPO13 Polyclonal antibody

• Featured Product
• KD/KO Validated

IPO13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11696-2-AP

Synonyms

KIAA0724, Karyopherin-13, Karyopherin 13, Kap13, Importin-13

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Validation Data Gallery

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  WB analysis using 11696-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11696-2-AP (IPO13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11696-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11696-2-AP (IPO13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11696-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11696-2-AP (IPO13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa cells using 11696-2-AP

  IF result in Hela cell from Dr. Ralph Kehlenbach showed Nuclear and Cytoplasma stain.

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  IF Staining of HeLa using 11696-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IPO13 antibody (11696-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, human brain tissue, Neura-2a cells, mouse lung
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

11696-2-AP targets IPO13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IPO13 fusion protein Ag2317
• Full Name: importin 13
• Calculated Molecular Weight: 963 aa, 108 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC008194
• Gene Symbol: IPO13
• Gene ID (NCBI): 9670
• RRID: AB_2127672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,IP	Nat Commun; Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response.; Authors - K A Gajewska ; KD Validated
human	WB,IHC,IF	Stem Cells; Importin 13 serves as a potential marker for corneal epithelial progenitor cells.; Authors - Wang Hua H
mouse	WB	Data Brief; Transcriptomic profile dataset of embryonic stem cells (Wild-type and IPO13-Knock Out) with and without oxidative stress.; Authors - Katarzyna A Gajewska ; KO Validated
human	WB	Cells; The Nuclear Transporter Importin 13 Can Regulate Stress-Induced Cell Death through the Clusterin/KU70 Axis; Authors - Katarzyna A Gajewska ; KD Validated