IHC staining of human colon cancer using 85651-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 85651-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 85651-1-RR
Immunofluorescent analysis of (4% PFA) fixed A431 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 85651-1-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells, A431 cells
Recommended dilution
Application
Dilution
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
85651-1-RR targets IQGAP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
IHC staining of human colon cancer using 85651-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 85651-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A431 using 85651-1-RR
Immunofluorescent analysis of (4% PFA) fixed A431 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 85651-1-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IQGAP1 Recombinant antibody
Catalog Number
85651-1-RR
Citations
-
Dilutions
IHC : 1:200-1:800 IF/ICC : 1:250-1:1000
Applications
IHC, IF/ICC, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
