IRE1; ERN1 Polyclonal antibody

IRE1; ERN1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

108

Cat no : 27528-1-AP

Synonyms

ERN1, IRE1/ERN1, hIRE1p, hIRE 1p, ERN 1

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Validation Data Gallery

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  WB analysis using 27528-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 27528-1-AP

  IP result of anti-IRE1; ERN1 (IP:27528-1-AP, 4ug; Detection:27528-1-AP 1:500) with A549 cells lysate 600 ug.

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  IHC staining of human pancreas cancer using 27528-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 27528-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of A549 using 27528-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human IRE1; ERN1 (27528-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, PC-3 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 101 publications below
• IHC: See 8 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

27528-1-AP targets IRE1; ERN1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRE1; ERN1 fusion protein Ag25580
• Full Name: endoplasmic reticulum to nucleus signaling 1
• Calculated Molecular Weight: 977 aa, 110 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC130405
• Gene Symbol: IRE1
• Gene ID (NCBI): 2081
• RRID: AB_2880899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

Publications

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang
	WB	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto
	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
rat	WB	Redox Biol; Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.; Authors - Yuehong Wang