Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, PC-3 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27528-1-AP targets IRE1/ERN1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25580 Product name: Recombinant human IRE1; ERN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 368-565 aa of BC130405 Sequence: PLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVI Predict reactive species |
| Full Name | endoplasmic reticulum to nucleus signaling 1 |
| Calculated Molecular Weight | 977 aa, 110 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC130405 |
| Gene Symbol | IRE1 |
| Gene ID (NCBI) | 2081 |
| RRID | AB_2880899 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75460 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IRE1/ERN1 antibody 27528-1-AP | Download protocol |
| IHC protocol for IRE1/ERN1 antibody 27528-1-AP | Download protocol |
| IP protocol for IRE1/ERN1 antibody 27528-1-AP | Download protocol |
| WB protocol for IRE1/ERN1 antibody 27528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
IRE1/ERN1 Polyclonal antibody (Cat# 27528-1-AP) has accumulated 196 citations across journals including Cell, Nature Communications, Cell Stem Cell, Cell Death & Disease, Redox Biology, Biomaterials, Free Radical Biology & Medicine, EMBO Journal, and FASEB Journal. Research fields encompass endoplasmic reticulum stress/unfolded protein response, oncology, neurology, cardiology, diabetes, hepatology, nephrology, immunology, toxicology, autophagy, ferroptosis, virology, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. | ||
Adv Mater Poly(Citric Acid) Bidirectional Regulator: Coordinating Iron Homeostasis to Suppress Stress Responses and Boosting Mitochondrial Bioenergetics for Enhanced Nerve Repair. | ||
Cell Stem Cell Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. | ||
Nat Commun Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity | ||
Nat Commun Natural photosynthetic system for restoring homeostasis of animal organelle interaction network. | ||
Redox Biol Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis |
