IRE1/ERN1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82687-5-RR (same clone as 82687-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82687-5-RR (IRE1; ERN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82687-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 82687-5-RR (same clone as 82687-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82687-5-RR (IRE1; ERN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82687-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82687-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82687-5-RR against Human IRE1/ERN1 were performed. The affinity constant is 1.12 nM.

IRE1/ERN1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 82687-5-PBS

Clone No.250145G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

ERN1, IRE1; ERN1, Endoplasmic reticulum-to-nucleus signaling 1, Endoribonuclease, ERN 1

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Product Information

82687-5-PBS targets IRE1/ERN1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag25580

  Product name: Recombinant human IRE1; ERN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 368-565 aa of BC130405

  Sequence: PLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVI

• Full Name: endoplasmic reticulum to nucleus signaling 1
• Calculated Molecular Weight: 977 aa, 110 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC130405
• Gene Symbol: IRE1
• Gene ID (NCBI): 2081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75460
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)