Validation Data Gallery
|Positive WB detected in||IFN gamma treated HeLa cells, Jurkat cells, HL-60 cells, Raji cells|
|Positive IP detected in||HL-60 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11335-1-AP targets IRF1 in WB, IP, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IRF1 fusion protein Ag1879|
|Full Name||IRF 1|
|Calculated molecular weight||325 aa, 37 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC009483|
|Gene ID (NCBI)||3659|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.
|Product Specific Protocols|
|WB protocol for IRF1 antibody 11335-1-AP||Download protocol|
|IHC protocol for IRF1 antibody 11335-1-AP||Download protocol|
|IP protocol for IRF1 antibody 11335-1-AP||Download protocol|
|FC protocol for IRF1 antibody 11335-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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