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IRF1 Polyclonal antibody

IRF1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11335-1-AP

Synonyms

IRF 1, IRF1, MAR



Tested Applications

Positive WB detected inIFN gamma treated HeLa cells, Jurkat cells, HL-60 cells, Raji cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11335-1-AP targets IRF1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF1 fusion protein Ag1879
Full Name IRF 1
Calculated molecular weight 325 aa, 37 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC009483
Gene symbol IRF1
Gene ID (NCBI) 3659
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IRF1 antibody 11335-1-APDownload protocol
IHC protocol for IRF1 antibody 11335-1-APDownload protocol
IP protocol for IRF1 antibody 11335-1-APDownload protocol
FC protocol for IRF1 antibody 11335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Manag Res

IRF1 inhibits the proliferation and metastasis of colorectal cancer by suppressing the RAS-RAC1 pathway.

Authors - Min Hong
humanWB

Nat Cell Biol

The RNA N6-methyladenosine modification landscape of human fetal tissues.

Authors - Shan Xiao
mouseWB,IHC

Am J Physiol Renal Physiol

PMID: 31091119

Authors - Zhiqiang Chen
ratWB

Biomed Res Int

Anti-Inflammatory and Antioxidant Effect of Eucommia ulmoides Polysaccharide in Hepatic Ischemia-Reperfusion Injury by Regulating ROS and the TLR-4-NF-κB Pathway.

Authors - Weidong Gao
pigWB

Arch Virol

PMID: 31385116

Authors - Lingling Shan
humanWB

Neurochem Res

LncRNA ZFAS1/miR-1271-5p/HK2 Promotes Glioma Development Through Regulating Proliferation, Migration, Invasion and Apoptosis.

Authors - Bin Zhang