IRF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IRF1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 11335-1-AP

Synonyms

IRF 1, IRF1, MAR

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Validation Data Gallery

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  WB analysis of HL-60 using 11335-1-AP

  WB result of IRF1 antibody (11335-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF1 transfected HL-60 cells.

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  WB analysis using 11335-1-AP

  Western blot analysis of HeLa cells, untreated or treated with Human IFN-γ (hIFN-γ) （HZ-1301） (100 ng/ml, 48 hr), using 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 11335-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11335-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11335-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11335-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11335-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 11335-1-AP

  IP result of anti-IRF1 (IP:11335-1-AP, 4ug; Detection:11335-1-AP 1:300) with HL-60 cells lysate 4400 ug.

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  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of Jurkat using 11335-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human IRF1 (11335-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Tested Applications

• Positive WB detected in: IFN gamma treated HeLa cells, Jurkat cells, HL-60 cells, Raji cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

11335-1-AP targets IRF1 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF1 fusion protein Ag1879
• Full Name: IRF 1
• Calculated molecular weight: 325 aa, 37 kDa
• Observed molecular weight: 45-50 kDa
• GenBank accession number: BC009483
• Gene symbol: IRF1
• Gene ID (NCBI): 3659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Publications

Species	Application	Title
human	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB,IHC,IF	Free Radic Biol Med; IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis; Authors - Yao Zhang ; KD Validated
human	WB	Front Immunol; Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells.; Authors - Hironori Bando
human	WB	Cancer Commun (Lond); MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.; Authors - Qingnan Wu
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li
human	WB	Front Immunol; PMID: 32373130; Authors - Karen Yotsumoto