IRF1 Polyclonal antibody

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  WB analysis of HL-60 using 11335-1-AP

  WB result of IRF1 antibody (11335-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF1 transfected HL-60 cells.

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  WB analysis using 11335-1-AP

  Western blot analysis of HeLa cells, untreated or treated with Human IFN-γ (hIFN-γ) （HZ-1301） (100 ng/ml, 48 hr), using 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11335-1-AP

  IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11335-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed IFN gamma treated HepG2 cells using IRF1 antibody (11335-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 11335-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human IRF1 (11335-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

• Featured Product
• KD/KO Validated

IRF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 11335-1-AP

Publications(45)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

MAR, IRF-1, IRF 1, interferon regulatory factor 1

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Tested Applications

• Positive WB detected in: IFN gamma treated HeLa cells, HL-60 cells, IFN gamma treated THP-1 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: IFN gamma treated HepG2 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 39 publications below
• IHC: See 8 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Product Information

11335-1-AP targets IRF1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF1 fusion protein Ag1879
• Full Name: IRF 1
• Calculated Molecular Weight: 325 aa, 37 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC009483
• Gene Symbol: IRF1
• Gene ID (NCBI): 3659
• RRID: AB_2877759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10914
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy; Authors - Chen Yang
	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	WB	J Immunother Cancer; Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors; Authors - Qian Tao
human	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
mouse	WB,IF	Cell Commun Signal; IRF1 regulation of ZBP1 links mitochondrial DNA and chondrocyte damage in osteoarthritis; Authors - Kai Sun ; KD Validated
human	WB,IHC,IF	Free Radic Biol Med; IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis; Authors - Yao Zhang ; KD Validated