IRF2BP1 Polyclonal antibody

IRF2BP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13698-1-AP

Synonyms

IRF 2 binding protein 1, IRF 2BP1, IRF2BP1

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Validation Data Gallery

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  WB analysis of HeLa using 13698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lymph using 13698-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13698-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13698-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13698-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13698-1-AP

  IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human breast cancer using 13698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13698-1-AP

  Immunofluorescent analysis of Hela cells, using IRF2BP1 antibody 13698-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat lymph tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

13698-1-AP targets IRF2BP1 in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF2BP1 fusion protein Ag4596
• Full Name: interferon regulatory factor 2 binding protein 1
• Calculated Molecular Weight: 584 aa, 62 kDa
• Observed Molecular Weight: 62-72 kDa
• GenBank Accession Number: BC038222
• Gene Symbol: IRF2BP1
• Gene ID (NCBI): 26145
• RRID: AB_2126853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF,ChIP	EMBO Rep; Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.; Authors - Sina V Barysch
human	WB	Cell Death Dis; The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.; Authors - Juan F Correa-Vázquez