IRF2BP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IRF2BP2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 18847-1-AP

Synonyms

IRF 2 binding protein 2, IRF 2BP2, IRF2BP2

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Validation Data Gallery

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  WB analysis of HeLa using 18847-1-AP

  WB result of IRF2BP2 antibody (18847-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF2BP2 transfected HeLa cells.

• 

  WB analysis of Jurkat using 18847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18847-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18847-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18847-1-AP

  IP result of anti-IRF2BP2 (IP:18847-1-AP, 3ug; Detection:18847-1-AP 1:500) with K-562 cells lysate 2400ug.

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  IHC staining of human lung cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18847-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

18847-1-AP targets IRF2BP2 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: interferon regulatory factor 2 binding protein 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: NM_182972
• Gene Symbol: IRF2BP2
• Gene ID (NCBI): 359948
• RRID: AB_10598163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.

Publications

Species	Application	Title
human	WB	Cancer Discov; Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.; Authors - Jana M Ellegast ; KO Validated
		Hepatology; The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop.; Authors - Xue Feng
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	ChIP	EMBO Rep; Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.; Authors - Sina V Barysch
mouse	WB,IHC	Hypertension; Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2).; Authors - Jing Fang ; KO Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher

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