Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18847-1-AP targets IRF2BP2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interferon regulatory factor 2 binding protein 2 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | NM_182972 |
| Gene Symbol | IRF2BP2 |
| Gene ID (NCBI) | 359948 |
| RRID | AB_10598163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z5L9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IHC protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IP protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| WB protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IRF2BP2 antibody (Cat# 18847-1-AP) has 17 citations published in journals including Signal Transduction and Targeted Therapy, Cancer Discovery, Nature Communications, Hepatology, Cell Death & Disease, Hypertension, EMBO Reports, Journal of Experimental Medicine, and others. Research fields span inflammation, cancer (AML, liver, gastric), SUMO modification, transcriptional regulation, cardiac hypertrophy, psoriasis, hypoxia, and endothelial biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Malate initiates a proton-sensing pathway essential for pH regulation of inflammation | ||
Cancer Discov Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.
| ||
Nat Commun IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation | ||
Hepatology The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop. | ||
Cell Death Dis The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis. |
