IRF5 Monoclonal antibody

IRF5 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C5

Cat no : 60088-1-Ig

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60088-1-Ig targets IRF5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IRF5 fusion protein Ag0841
Full Name interferon regulatory factor 5
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 63+110 kDa
GenBank Accession NumberBC004201
Gene Symbol IRF5
Gene ID (NCBI) 3663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. I t is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10).

Protocols

Product Specific Protocols
WB protocol for IRF5 antibody 60088-1-IgDownload protocol
IHC protocol for IRF5 antibody 60088-1-IgDownload protocol
IF protocol for IRF5 antibody 60088-1-IgDownload protocol
IP protocol for IRF5 antibody 60088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Manipulation of the toll-like receptor 7 signaling pathway by Epstein-Barr virus.

Authors - Martin Heather J HJ