IRF7 Recombinant antibody

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  WB analysis using 85072-5-RR

  IFN alpha treated and untreated Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85072-5-RR (IRF7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 85072-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug IRF7 Recombinant antibody (85072-5-RR, Clone:242613C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85072-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85072-5-RR against Human IRF7 were performed. The affinity constant is 13.1 pM.

IRF7 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 85072-5-RR

Clone No.242613C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

242613C2, IRF 7, IRF 7H, IRF-7, IRF7A

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Tested Applications

• Positive WB detected in: IFN alpha treated Jurkat cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85072-5-RR targets IRF7 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IRF7 fusion protein Ag18059
• Full Name: IRF 7
• Calculated Molecular Weight: 516 aa, 56 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC136555
• Gene Symbol: IRF7
• Gene ID (NCBI): 3665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92985
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).