Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24639-1-AP targets ISG20L2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20026 Product name: Recombinant human ISG20L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC000575 Sequence: MSTLLLNLDFGEPPPKKALEGNAKHRNFVKKRRLLERRGFLSKKNQPPSKAPKLHSEPSKKGETPTVDGTWKTPSFPKKKTAASSNGSGQPLDKKAAVSWLTPAPSKKADSVAAKVDLLGEFQSALPKINSHPTRSQKKSSQKKSSKKNHPQKNAPQNSTQAHSENKCSGASQKLPRKMVAIDCEMVGTGPKGHVSSLARCSIVNYNGDVLYDEYILPPCHIVDYRTRWSGIRKQHMVNATPFKIARGQILKILTGKIVVGHAIHNDFKALQYFHPKSLTRDTSHIPPLNRKADCPENATMSLKHLTKKLLNRDIQVGKSGHSSVEDAQATMELYKLVEVEWEEHLARNPPTD Predict reactive species |
| Full Name | interferon stimulated exonuclease gene 20kDa-like 2 |
| Calculated Molecular Weight | 353 aa, 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC000575 |
| Gene Symbol | ISG20L2 |
| Gene ID (NCBI) | 81875 |
| RRID | AB_2879649 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9L3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ISG20L2, also named HSD38, is a member of a vertebrate exonucleases family. ISG20L2 is composed of at least two functional domains, one at its N-terminal half allowing intracellular localization and association with the binding partners and the other at its C-terminal half bearing the 3` to 5` exoribonuclease activity. The molecular weight of ISG20L2 is 39 kDa (PMID: 18065403).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ISG20L2 antibody 24639-1-AP | Download protocol |
| IP protocol for ISG20L2 antibody 24639-1-AP | Download protocol |
| WB protocol for ISG20L2 antibody 24639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ISG20L2 Polyclonal antibody (Cat# 24639-1-AP) has 5 citations published in Molecular Cell, Nucleic Acids Research (×2), JCI Insight, and bioRxiv. The cited research spans RNA processing and ribosome biogenesis (18S/28S rRNA maturation, ITS1 cleavage), exonuclease-mediated R-loop resolution and anti-tumor immunity, bortezomib resistance in myeloma, and proteome-wide C-degron/CTLH E3 ligase profiling linked to ribosome biogenesis. Applications cited include WB and IP in human samples.
| Species | Application | Title |
|---|---|---|
Mol Cell RNA exonuclease REXO4 resolves m(6)A-marked R-loops and suppresses anti-tumor immunity. | ||
Nucleic Acids Res Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA | ||
Nucleic Acids Res The 3'-5' exoribonuclease ISG20L2 contributes to 3' terminus maturation of 18S and 28S ribosomal RNAs.
| ||
JCI Insight ISG20L2 suppresses bortezomib anti-myeloma activity by attenuating bortezomib binding to PSMB5 | ||
bioRxiv Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis. |
Reviews
What customers say
The single review rates this ISG20L2 antibody 5 out of 5 stars, calling it the best ISG20L2 reagent the reviewer has tried compared to other commercial options. It performed well for Western blot (1:5000 dilution) and immunoprecipitation. However, the reviewer notes it did not work properly for immunofluorescence, at least in Jurkat T cells. Overall, the feedback is strongly positive for WB and IP applications, with a caveat regarding IF performance in that specific cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sara (Verified Customer) (05-24-2023) | It is the best ISG20L2 I have tried by far compared to other commercial ones. I have used it for WB (1:5000) and for immunoprecipitation. I need to mention that we tried it for IF but it did not work properly, at least for Jurkat T cells!
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