Islet 1 Polyclonal antibody

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  WB analysis of BxPC-3 using 15661-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15661-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15661-1-AP

  IP result of anti-Islet 1 (IP:15661-1-AP, 4ug; Detection:15661-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human pancreas cancer using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of SH-SY5Y using 15661-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (15661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 15661-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Islet 1 (15661-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Islet 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15661-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 3 publications below

Product Information

15661-1-AP targets Islet 1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8088

  Product name: Recombinant human Islet 1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-349 aa of BC031213

  Sequence: MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA

• Full Name: ISL LIM homeobox 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC031213
• Gene Symbol: Islet 1
• Gene ID (NCBI): 3670
• RRID: AB_2126321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61371
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu
human		ACS Chem Neurosci; Amyotrophic Lateral Sclerosis-Associated Persistent Organic Pollutant cis-Chlordane Causes GABAA-Independent Toxicity to Motor Neurons, Providing Evidence toward an Environmental Component of Sporadic Amyotrophic Lateral Sclerosis; Authors - Daniel Kulick
human	IF	Sci Prog; Incorporation of decellularized-ECM in graphene-based scaffolds enhances axonal outgrowth and branching in neuro-muscular co-cultures; Authors - Carlos Serna

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-10-2019)

ISL1 antibody recognizes a couple bands above 50kD which is a bit off from the expected molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Islet 1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15661-1-AP)