Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15661-1-AP targets Islet 1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8088 Product name: Recombinant human Islet 1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-349 aa of BC031213 Sequence: MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA Predict reactive species |
| Full Name | ISL LIM homeobox 1 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39-45 kDa |
| GenBank Accession Number | BC031213 |
| Gene Symbol | Islet 1 |
| Gene ID (NCBI) | 3670 |
| RRID | AB_2126321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61371 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Islet 1 antibody 15661-1-AP | Download protocol |
| IF protocol for Islet 1 antibody 15661-1-AP | Download protocol |
| IHC protocol for Islet 1 antibody 15661-1-AP | Download protocol |
| IP protocol for Islet 1 antibody 15661-1-AP | Download protocol |
| WB protocol for Islet 1 antibody 15661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Islet 1 (ISL1) polyclonal antibody (Cat# 15661-1-AP) has 7 citations published in Cells, ACS Chemical Neuroscience, Journal of Biological Chemistry, Neuroscience, Frontiers in Neurology, Science Progress, and Science China Life Sciences. The cited research spans neuroscience (neurotoxicity, cerebral organoids, spinal cord injury), stem cell biology (pluripotent and embryonic stem cell differentiation), and cardiology (cardiomyocyte development).
| Species | Application | Title |
|---|---|---|
Cells Generation of Functional GnRH-Responsive Pituitary-like Organoids from Ovine Embryonic Stem Cells. | ||
ACS Chem Neurosci Amyotrophic Lateral Sclerosis-Associated Persistent Organic Pollutant cis-Chlordane Causes GABAA-Independent Toxicity to Motor Neurons, Providing Evidence toward an Environmental Component of Sporadic Amyotrophic Lateral Sclerosis | ||
J Biol Chem Ginkgolide A Enhances Cardiomyocyte Differentiation from Pluripotent Stem Cells by Targeting Cytochrome c to Attenuate Intrinsic Apoptosis. | ||
Front Neurol Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model. | ||
Sci Prog Incorporation of decellularized-ECM in graphene-based scaffolds enhances axonal outgrowth and branching in neuro-muscular co-cultures |
Reviews
What customers say
The sole review (rated 1/5) was posted by Joleen Cheah in June 2019 for Western Blot on MDCK GII cells at a 1:1,000 dilution. She reported that the ISL1 antibody detected bands above 50 kD, which she noted was slightly off from the expected molecular weight of the target protein. Overall, the reviewer expressed dissatisfaction with the specificity of the band pattern observed.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (06-10-2019) | ISL1 antibody recognizes a couple bands above 50kD which is a bit off from the expected molecular weight.
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