Islet 1 Polyclonal antibody

Islet 1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 15661-1-AP


Isl 1, ISL LIM homeobox 1, ISL1, Islet 1, ISLET1

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

15661-1-AP targets Islet 1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Islet 1 fusion protein Ag8088
Full Name ISL LIM homeobox 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa, 45 kDa
GenBank Accession NumberBC031213
Gene Symbol ISL1
Gene ID (NCBI) 3670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.


Product Specific Protocols
WB protocol for Islet 1 antibody 15661-1-APDownload protocol
IHC protocol for Islet 1 antibody 15661-1-APDownload protocol
IF protocol for Islet 1 antibody 15661-1-APDownload protocol
IP protocol for Islet 1 antibody 15661-1-APDownload protocol
FC protocol for Islet 1 antibody 15661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Neurol

Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.

Authors - Jing Huang


PMID: 30599272

Authors - Fangkun Liu

ACS Chem Neurosci

Amyotrophic Lateral Sclerosis-Associated Persistent Organic Pollutant cis-Chlordane Causes GABAA-Independent Toxicity to Motor Neurons, Providing Evidence toward an Environmental Component of Sporadic Amyotrophic Lateral Sclerosis

Authors - Daniel Kulick


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Joleen (Verified Customer) (06-10-2019)

ISL1 antibody recognizes a couple bands above 50kD which is a bit off from the expected molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Islet 1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15661-1-AP)