Islet 1 Polyclonal antibody

Islet 1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 15661-1-AP

Synonyms

Isl 1, ISL LIM homeobox 1, ISL1, Islet 1, ISLET1

---

Validation Data Gallery

• 

  WB analysis of BxPC-3 using 15661-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15661-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15661-1-AP

  IP result of anti-Islet 1 (IP:15661-1-AP, 4ug; Detection:15661-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human pancreas using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 15661-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (15661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 15661-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Islet 1 (15661-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

---

Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

15661-1-AP targets Islet 1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Islet 1 fusion protein Ag8088
• Full Name: ISL LIM homeobox 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa, 45 kDa
• GenBank Accession Number: BC031213
• Gene Symbol: ISL1
• Gene ID (NCBI): 3670
• RRID: AB_2126321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.

Publications

Species	Application	Title
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu
human		ACS Chem Neurosci; Amyotrophic Lateral Sclerosis-Associated Persistent Organic Pollutant cis-Chlordane Causes GABAA-Independent Toxicity to Motor Neurons, Providing Evidence toward an Environmental Component of Sporadic Amyotrophic Lateral Sclerosis; Authors - Daniel Kulick

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Joleen (Verified Customer) (06-10-2019)

ISL1 antibody recognizes a couple bands above 50kD which is a bit off from the expected molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Islet 1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15661-1-AP)