ISLR2 Polyclonal antibody

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  WB analysis using 24814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24814-1-AP (ISLR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ISLR2 Polyclonal Antibody for WB, ELISA

Cat No. 24814-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Immunoglobulin superfamily containing leucine-rich repeat protein 2, KIAA1465, Leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein, LINX

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24814-1-AP targets ISLR2 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20518

  Product name: Recombinant human ISLR2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 396-745 aa of BC152429

  Sequence: ERKSTAKGRGNSVLPSKPEGKIKGQGLAKVSILGETETEPEEDTSEGEEAEDQILADPAEEQRCGNGDPSRYVSNHAFNQSAELKPHVFELGVIALDVAEREARVQLTPLAARWGPGPGGAGGAPRPGRRPLRLLYLCPAGGGAAVQWSRVEEGVNAYWFRGLRPGTNYSVCLALAGEACHVQVVFSTKKELPSLLVIVAVSVFLLVLATVPLLGAACCHLLAKHPGKPYRLILRPQAPDPMEKRIAADFDPRASYLESEKSYPAGGEAGGEEPEDVQGEGLDEDAEQGDPSGDLQREESLAACSLVESQSKANQEEFEAGSEYSDRLPLGAEAVNIAQEINGNYRQTAG

• Full Name: immunoglobulin superfamily containing leucine-rich repeat 2
• Calculated Molecular Weight: 745 aa, 79 kDa
• Observed Molecular Weight: 79 kDa, 130 kDa
• GenBank Accession Number: BC152429
• Gene Symbol: ISLR2
• Gene ID (NCBI): 57611
• RRID: AB_3669451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UXK2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISLR2 (immunoglobulin superfamily containing leucine rich repeat 2), also known as LINX. It is expected to be located in cell membrane and the protein is mainly expressed in brain and testis. While the first knockout mouse had impaired development of the internal capsule and no hydrocephalus, a much more recent independent knockout mouse was shown to additionally have severe hydrocephalus. Although the mechanism remains unclear, it may be related to the role of ISLR2 in reorganizing the cytoskeleton of developing neurons (PMID: 30483960). The calculated molecular weight of ISLR2 is 79 kDa, this target has phosphorylation modification and glycosylation modification. And the 130 kDa band is glycosylated (PMID: 29739947).