ITGA6X1A Polyclonal antibody

ITGA6X1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20231-1-AP

Synonyms

CD49f, Integrin alpha 6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

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Validation Data Gallery

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  WB analysis of mouse spleen using 20231-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20231-1-AP (ITGA6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20231-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20231-1-AP (ITGA6-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20231-1-AP targets ITGA6X1A in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, alpha 6
• Calculated Molecular Weight: 127 kDa
• Observed Molecular Weight: 100 kDa, 150 kDa
• GenBank Accession Number: NM_000210
• Gene Symbol: Integrin alpha 6
• Gene ID (NCBI): 3655
• RRID: AB_10665420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGA6, also named as CD49f and VLA-6, belongs to the integrin alpha chain family. It is a receptor for laminin on platelets. ITGA6 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Defects in ITGA6 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) which also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. The antibody is specific to isoform Alpha-6X1A, isoform Alpha-6X2A, isoform Alpha-6X1X2A isoform7 and isoform9 of ITGA6.