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Integrin Beta 4 Polyclonal antibody

Integrin Beta 4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25277-1-AP

Synonyms

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    WB analysis of BxPC-3 using 25277-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    WB analysis of rat skin using 25277-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse pancreas tissue using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    WB analysis of mouse pancreas using 25277-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    IHC staining of human breast cancer using 25277-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    IHC staining of human breast cancer using 25277-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    IHC staining of human pancreas cancer using 25277-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    IHC staining of human pancreas cancer using 25277-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Integrin Beta 4 Polyclonal antibody (25277-1-AP)

    IF Staining of A431 using 25277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Integrin Beta 4 antibody (25277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, mouse pancreas tissue, rat skin tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25277-1-AP targets Integrin Beta 4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Beta 4 fusion protein Ag16460
Full Name integrin, beta 4
Calculated Molecular Weight 1752 aa, 195 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC126411
Gene Symbol ITGB4
Gene ID (NCBI) 3691
RRIDAB_2880002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 4 antibody 25277-1-APDownload protocol
IHC protocol for Integrin Beta 4 antibody 25277-1-APDownload protocol
IF protocol for Integrin Beta 4 antibody 25277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

A mutation found in esophageal cancer alters integrin β4 mRNA splicing.

Authors - Gabriel T Kelly
View Article