Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse pancreas tissue, rat skin tissue |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25277-1-AP targets Integrin beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16460 Product name: Recombinant human ITGB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1411-1752 aa of BC126411 Sequence: VLSTSSTLTRDYNSLTRSEHSHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEFVSRTLTTSGTLSTHMDQQFFQT Predict reactive species |
| Full Name | integrin, beta 4 |
| Calculated Molecular Weight | 1752 aa, 195 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC126411 |
| Gene Symbol | Integrin beta 4 |
| Gene ID (NCBI) | 3691 |
| RRID | AB_2880002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Integrin beta 4 antibody 25277-1-AP | Download protocol |
| IHC protocol for Integrin beta 4 antibody 25277-1-AP | Download protocol |
| WB protocol for Integrin beta 4 antibody 25277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Integrin beta 4 Polyclonal antibody (Cat# 25277-1-AP) has 2 citations. One appears in Biochemical and Biophysical Research Communications (2020), studying integrin β4 mRNA splicing mutations in esophageal cancer via Western blot in human samples. The other is in Journal of Oral Biosciences (2024), examining gingival junctional epithelial cell adhesion on nanocomposite surfaces using immunofluorescence in mouse models. The associated research fields span cancer biology (esophageal carcinoma) and oral/dental biology (cell adhesion).
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun A mutation found in esophageal cancer alters integrin β4 mRNA splicing. | ||
J Oral Biosci Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion |
