Integrin Beta 4 Polyclonal antibody

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  WB analysis of BxPC-3 using 25277-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 25277-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of mouse pancreas using 25277-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25277-1-AP (ITGB4 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IHC staining of human breast cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 25277-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25277-1-AP (ITGB4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 25277-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Integrin Beta 4 antibody (25277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Integrin Beta 4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25277-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse pancreas tissue, rat skin tissue
• Positive IHC detected in: human breast cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

25277-1-AP targets Integrin Beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16460

  Product name: Recombinant human ITGB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1411-1752 aa of BC126411

  Sequence: VLSTSSTLTRDYNSLTRSEHSHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEFVSRTLTTSGTLSTHMDQQFFQT

• Full Name: integrin, beta 4
• Calculated Molecular Weight: 1752 aa, 195 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC126411
• Gene Symbol: Integrin beta 4
• Gene ID (NCBI): 3691
• RRID: AB_2880002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16144
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochem Biophys Res Commun; A mutation found in esophageal cancer alters integrin β4 mRNA splicing.; Authors - Gabriel T Kelly
mouse	IF	J Oral Biosci; Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion; Authors - Shoma Yamamori