ITIH3 Polyclonal antibody

ITIH3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21247-1-AP

Synonyms

Serum-derived hyaluronan-associated protein, ITI-HC3, ITI heavy chain H3, ITI HC3, Inter-alpha-trypsin inhibitor heavy chain H3

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Validation Data Gallery

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  WB analysis of human plasma using 21247-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 21247-1-AP (ITIH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 21247-1-AP

  IP result of anti-ITIH3 (IP:21247-1-AP, 4ug; Detection:21247-1-AP 1:1000) with human plasma lysate 4000ug.

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  IHC staining of human stomach cancer using 21247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 21247-1-AP (ITIH3 Antibody) at dilution of 1:50.

• 

  IHC staining of human stomach cancer using 21247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 21247-1-AP (ITIH3 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: human serum tissue
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

21247-1-AP targets ITIH3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITIH3 fusion protein Ag15750
• Full Name: inter-alpha (globulin) inhibitor H3
• Calculated Molecular Weight: 890 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC107604
• Gene Symbol: ITIH3
• Gene ID (NCBI): 3699
• RRID: AB_2878830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITIH3, or inter-alpha-trypsin inhibitor heavy chain 3, is a gene in humans that encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex plays a role in stabilizing the extracellular matrix through its ability to bind hyaluronic acid. The gene is located on chromosome 3 and is part of the inter-alpha-trypsin inhibitor family gene cluster. ITIH3 has restricted expression primarily toward the liver, with an RPKM value of 228.8, indicating a relatively high level of expression in this organ.

Publications

Species	Application	Title
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
human	WB	J Pers Med; Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer.; Authors - Jing Yu
	WB	Sci Rep; Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.; Authors - Chang Zhao
human	IHC	Dis Markers; Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.; Authors - Xiao Jiang