ITLN1/2 Polyclonal antibody

ITLN1/2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 11770-1-AP

Synonyms

ITLN1, HL 1, hIntL, Galactofuranose-binding lectin, Galactofuranose binding lectin

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Validation Data Gallery

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  WB analysis of mouse colon using 11770-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11770-1-AP (ITLN1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 11770-1-AP

  IP result of anti-ITLN1/2 (IP:11770-1-AP, 4ug; Detection:11770-1-AP 1:500) with mouse appendix tissue lysate 4000ug.

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  IHC staining of human stomach using 11770-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11770-1-AP (ITLN1/2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IP detected in: mouse appendix tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Product Information

11770-1-AP targets ITLN1/2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITLN1/2 fusion protein Ag2424
• Full Name: intelectin 1 (galactofuranose binding)
• Calculated Molecular Weight: 313 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC020664
• Gene Symbol: ITLN1
• Gene ID (NCBI): 55600
• RRID: AB_2129677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITLN1, also named as Intelectin 1, has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. ITLN1 is a glycoprotein made up of three 40 kDa subunits cross-linked by disulfide bonds making up a 120-kDa homo-trimer of 295 amino acids and N-linked oligosaccharides (PMID: 17621593). It may play a role in the defense system against microorganisms. Human intelectin-1 is expressed in intestinal goblet cells, and is present at about 200 ng/mL in serum. Intelectin-1 was also shown to be expressed in human endothelial cells and adipocytes of human abdominal adipose tissue. (PMID: 22768319,PMID: 28640441)

Publications

Species	Application	Title
	IF	Nat Commun; Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates.; Authors - Hye Young Kim
human	IHC, IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB,IHC	Mucosal Immunol; Intelectin contributes to allergen-induced IL-25, IL-33, and TSLP expression and type 2 response in asthma and atopic dermatitis.; Authors - L Yi
mouse	WB,IF	Front Cell Dev Biol; Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells.; Authors - Xiaochen Niu
human	WB	Fish Shellfish Immunol; Molecular properties and ligand specificity of zebrafish intelectin-2.; Authors - Nongnuch Singrang
human	WB	J Biol Chem; Structures of Xenopus embryonic epidermal lectin reveal a conserved mechanism of microbial glycan recognition.; Authors - Kittikhun Wangkanont