CoraLite®594-conjugated ITM2C Monoclonal antibody

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  IF Staining of MDCK using CL594-60095

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using the CoraLite®594-conjugated version of this antibody, CL594-60095 (ITM2C antibody), at dilution of 1:100.

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  IF Staining of HepG2 using CL594-60095

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 ITM2C antibody (CL594-60095, Clone: 2E8G11 ) at dilution of 1:200.

ITM2C Monoclonal Antibody for IF/ICC

Cat No. CL594-60095

Clone No.2E8G11

Host / Isotype

Mouse / IgG2a

Reactivity

human, canine

Applications

IF/ICC

Synonyms

E25, CT-BRI3, Cerebral protein 14, BRICD2C, BRI3

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60095 targets ITM2C in IF/ICC applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4409

  Product name: Recombinant human ITM2C protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-230 aa of BC025742

  Sequence: MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGSSVGGVCYLSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLYEDSLSSQVRTQMELEEDVKIYLDENYERINVPVPQFGGGDPADIIHDFQRRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRRRATRRRINKRGAKNCNAIRHFENTFVVETLICGVV

• Full Name: integral membrane protein 2C
• Calculated Molecular Weight: 230aa,26 kDa; 267aa,30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC025742
• Gene Symbol: ITM2C
• Gene ID (NCBI): 81618
• RRID: AB_2883421
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQX7
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Integral membrane protein 2C (ITM2C) is a type II integral transmembrane protein belonging to a family composed of at least two other members, ITM2A and ITM2B. ITM2C, often called transmembrane protein BRI3, is highly expressed in brain tissue. Yeast two-hybrid screen system revealed that BRI3 could interact with beta-secretase beta-amyloid protein converting enzyme (BACE)1 and the microtubule-destabilizing protein SCG10 (STMN2). It may play a role in TNF-induced cell death and neuronal differentiation. BRI3 was found to inhibit the various processing of amyloid precursor protein (APP) by blocking the access of alpha- and beta-secretases to APP, competitive inhibition of APP processing by BRI3 may provide a new approach to Alzheimer disease (AD) therapy and prevention.