ITPA Polyclonal antibody

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• ITPA Polyclonal antibody (16134-1-AP)

ITPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16134-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Non-canonical purine NTP pyrophosphatase, My049, ITPase, HLC14 06 P, EC:3.6.1.9

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  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 16134-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 16134-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 16134-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 16134-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 16134-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16134-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16134-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 16134-1-AP

  IP result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 16134-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human heart tissue, human lung cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16134-1-AP targets ITPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9128

  Product name: Recombinant human ITPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-194 aa of BC010138

  Sequence: MAASLVGKKIVFVTGNAKKLEEVVQILGDKFPCTLVAQKIDLPEYQGEPDEISIQKCQEAVRQVQGPVLVEDTCLCFNALGGLPGPYIKWFLEKLKPEGLHQLLAGFEDKSAYALCTFALSTGDPSQPVRLFRGRTSGRIVAPRGCQDFGWDPCFQPDGYEQTYAEMPKAEKNAVSHRFRALLELQEYFGSLAA

• Full Name: inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• Calculated Molecular Weight: 194 aa, 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC010138
• Gene Symbol: ITPA
• Gene ID (NCBI): 3704
• RRID: AB_2129821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY32
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.

Publications

What published studies show

The ITPA Polyclonal antibody (Cat# 16134-1-AP) has 4 total citations. These appear in Frontiers in Immunology, Biochimie, Biochimica et Biophysica Acta, and Oncotarget. The associated research fields span neuroscience (glial remodeling in visual cortex), biochemistry (ITPase nucleobase specificity and mutant characterization), and oncology (renal-cell carcinoma survival prediction). Applications cited include Western blot and immunohistochemistry.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Front Immunol; Single-cell sequencing reveals reversible glial remodeling in the visual cortex during visual deprivation and recovery.; Authors - Xiaoqi Gong
human	WB	Biochimie; Analysis of human ITPase nucleobase specificity by site-directed mutagenesis.; Authors - Hill Amanda A
human	IHC	Oncotarget; A four-gene signature predicts survival in clear-cell renal-cell carcinoma.; Authors - Jun Dai
human	WB	Biochim Biophys Acta; Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.; Authors - Herting Greg G