ITPKC Polyclonal antibody, PBS Only

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• ITPKC Polyclonal antibody, PBS Only (14267-1-PBS)

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  WB analysis using 14267-1-AP (same clone as 14267-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

  IP result of anti-ITPKC (IP:14267-1-AP, 4ug; Detection:14267-1-AP 1:400) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ITPKC antibody 14267-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

ITPKC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14267-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

14267-1-PBS targets ITPKC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5583

  Product name: Recombinant human ITPKC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-358 aa of BC060788

  Sequence: MRRCPCRGSLNEAEAGALPAAARMGLEAPRGGRRRQPGQQRPGPGAGAPAGRPEGGGPWARTEGSSLHSEPERAGLGPAPGTESPQAEFWTDGQTEPAAAGLGVETERPKQKTEPDRSSLRTHLEWSWSELETTCLWTETGTDGLWTDPHRSDLQFQPEEASPWTQPGVHGPWTELETHGSQTQPERVKSWADNLWTHQNSSSLQTHPEGACPSKEPSADGSWKELYTDGSRTQQDIEGPWTEPYTDGSQKKQDTEAARKQPGTGGFQIQQDTDGSWTQPSTDGSQTAPGTDCLLGEPEDGPLEEPEPGELLTHLYSHLKCSPLCPVPRLIITPETPEPEAQPVGPPSRVEGGSGGFS

• Full Name: inositol 1,4,5-trisphosphate 3-kinase C
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC060788
• Gene Symbol: ITPKC
• Gene ID (NCBI): 80271
• RRID: AB_2280728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96DU7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).