ITPR1-Specific Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85101-1-RR (same clone as 85101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85101-1-RR (ITPR1-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85101-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85101-1-RR (same clone as 85101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85101-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85101-1-RR (same clone as 85101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85101-1-PBS in a different storage buffer formulation.

ITPR1-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85101-1-PBS

Clone No.242274B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ITPR1, 242274B10, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1

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Product Information

85101-1-PBS targets ITPR1-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inositol 1,4,5-triphosphate receptor, type 1
• Calculated Molecular Weight: 314 kDa
• Observed Molecular Weight: 290-300 kDa
• GenBank Accession Number: NM_001099952
• Gene Symbol: ITPR1
• Gene ID (NCBI): 3708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14643
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.