ITPR1-Specific Recombinant monoclonal antibody

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  WB analysis using 85101-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85101-1-RR (ITPR1-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85101-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 85101-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITPR1-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85101-1-RR

Clone No.242274B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ITPR1, 242274B10, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, L02 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85101-1-RR targets ITPR1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inositol 1,4,5-triphosphate receptor, type 1
• Calculated Molecular Weight: 314 kDa
• Observed Molecular Weight: 290-300 kDa
• GenBank Accession Number: NM_001099952
• Gene Symbol: ITPR1
• Gene ID (NCBI): 3708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14643
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.