Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, human liver tissue, Jurkat cells, L02 cells |
| Positive IHC detected in | human liver cancer tissue, mouse lung tissue, human liver tissue, mouse liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66032-1-Ig targets IVD in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18042 Product name: Recombinant human IVD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-423 aa of BC017202 Sequence: MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH Predict reactive species |
| Full Name | isovaleryl Coenzyme A dehydrogenase |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 41-46 kDa |
| GenBank Accession Number | BC017202 |
| Gene Symbol | IVD |
| Gene ID (NCBI) | 3712 |
| RRID | AB_11042768 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26440 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IVD antibody 66032-1-Ig | Download protocol |
| IHC protocol for IVD antibody 66032-1-Ig | Download protocol |
| WB protocol for IVD antibody 66032-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IVD (Isovaleryl CoA dehydrogenase, mitochondrial) monoclonal antibody (clone 2D12A5), catalog number 66032-1-Ig, has 1 total citation. It appears in Archives of Toxicology (Impact Factor 10.9), published in July 2015. The cited study investigated how activation of PPARα ameliorates perfluorododecanoic acid–induced reactive oxygen species production in rat liver, using Western blot. The research field encompasses toxicology, oxidative stress, and hepatic metabolism.
| Species | Application | Title |
|---|---|---|
Arch Toxicol Activation of peroxisome proliferator-activated receptor α ameliorates perfluorododecanoic acid-induced production of reactive oxygen species in rat liver. |
