IVD Monoclonal antibody, PBS Only

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  WB analysis using 66032-1-Ig (same clone as 66032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66032-1-Ig (same clone as 66032-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using IVD antibody 66032-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

IVD Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 66032-1-PBS

Clone No.2D12A5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

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Product Information

66032-1-PBS targets IVD in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18042

  Product name: Recombinant human IVD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-423 aa of BC017202

  Sequence: MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH

• Full Name: isovaleryl Coenzyme A dehydrogenase
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 41-46 kDa
• GenBank Accession Number: BC017202
• Gene Symbol: IVD
• Gene ID (NCBI): 3712
• RRID: AB_11042768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26440
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.