IZUMO1 Polyclonal antibody

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  WB analysis using 13296-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13296-1-AP (IZUMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 13296-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 13296-1-AP (IZUMO1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IZUMO1 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 13296-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

IZUMO, Izumo sperm-egg fusion protein 1, OBF, Sperm-specific protein izumo

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13296-1-AP targets IZUMO1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4008

  Product name: Recombinant human IZUMO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 31-294 aa of BC034769

  Sequence: LALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLL

• Full Name: izumo sperm-egg fusion 1
• Calculated Molecular Weight: 350 aa, 39 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC034769
• Gene Symbol: IZUMO1
• Gene ID (NCBI): 284359
• RRID: AB_2877934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Izumo sperm egg fusion gene (IZUMO1) is a crucial member of the immunoglobulin superfamily and plays a crucial role in spermatogenesis, sperm egg interaction, and fusion. The expression of IZUMO1 mRNA was mainly in the testis and sperm and performed an important part in fertilization. IZUMO1 possess a well-conserved N-glycosylation site in the middle of an Ig loop among species. Research indicates that the glycosylated form of IZUMO1 has a molecular weight of 56 kDa, while its dimeric form has a molecular weight of 70 kDa (PMID: 21057513, PMID: 31146500).