IZUMO1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• IZUMO1 Polyclonal antibody (13296-1-AP)

IZUMO1 Polyclonal Antibody for WB, ELISA

Cat No. 13296-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IZUMO, Izumo sperm-egg fusion protein 1, OBF, Sperm-specific protein izumo

• 

  WB analysis using 13296-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13296-1-AP (IZUMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for IZUMO1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13296-1-AP targets IZUMO1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4008

  Product name: Recombinant human IZUMO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 31-294 aa of BC034769

  Sequence: LALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLL

• Full Name: izumo sperm-egg fusion 1
• Calculated Molecular Weight: 350 aa, 39 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC034769
• Gene Symbol: IZUMO1
• Gene ID (NCBI): 284359
• RRID: AB_2877934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Izumo sperm egg fusion gene (IZUMO1) is a crucial member of the immunoglobulin superfamily and plays a crucial role in spermatogenesis, sperm egg interaction, and fusion. The expression of IZUMO1 mRNA was mainly in the testis and sperm and performed an important part in fertilization. IZUMO1 possess a well-conserved N-glycosylation site in the middle of an Ig loop among species. Research indicates that the glycosylated form of IZUMO1 has a molecular weight of 56 kDa, while its dimeric form has a molecular weight of 70 kDa (PMID: 21057513, PMID: 31146500).

Publications

What published studies show

The IZUMO1 Polyclonal antibody (Cat# 13296-1-AP) has 1 citation. It was cited in Molecular Cell (IF 16), in a study on chaperonin TRiC and sperm flagellar motility, using the antibody for Western blot in mouse samples. The research field is reproductive biology and cell biology, specifically related to sperm function and protein folding in mammalian flagella.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell; Chaperonin TRiC bridges radial spokes for folding locally translated proteins to sustain mammalian sperm flagellar motility.; Authors - Xueming Meng