IZUMO1 Polyclonal antibody

IZUMO1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13296-1-AP

Synonyms

IZUMO, izumo sperm egg fusion 1, IZUMO1, OBF, Oocyte binding/fusion factor, Sperm specific protein izumo



Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13296-1-AP targets IZUMO1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IZUMO1 fusion protein Ag4008
Full Name izumo sperm-egg fusion 1
Calculated Molecular Weight 350 aa, 39 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC034769
Gene Symbol IZUMO1
Gene ID (NCBI) 284359
RRIDAB_2877934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IZUMO1, also named as OBF, is a sperm-specific protein. It is a novel member of the immunoglobulin superfamily located on sperm and is indispensable for sperm-egg fusion. For mouse Izumo1, the MW is about 56 kDa. For human IZUMO1, the MW is about 37-40 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for IZUMO1 antibody 13296-1-APDownload protocol
IHC protocol for IZUMO1 antibody 13296-1-APDownload protocol
IF protocol for IZUMO1 antibody 13296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols