CoraLite® Plus 488-conjugated Iduronate 2 sulfatase Monoclonal antibody

Iduronate 2 sulfatase Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat , pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3F9

Cat no : CL488-66112

Synonyms

IDS, iduronate 2 sulfatase, Idursulfase, MPS2, SIDS

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66112

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Iduronate 2 sulfatase antibody (CL488-66112, Clone: 1A3F9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66112 targets Iduronate 2 sulfatase in IF applications and shows reactivity with human, mouse, rat , pig samples.

• Tested Reactivity: human, mouse, rat , pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Iduronate 2 sulfatase fusion protein Ag19095
• Full Name: iduronate 2-sulfatase
• Calculated Molecular Weight: 312aa,35 kDa; 550aa,62 kDa
• Observed Molecular Weight: 76 kDa, 55 kDa
• GenBank Accession Number: BC006170
• Gene Symbol: IDS
• Gene ID (NCBI): 3423
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).