PE Anti-Human IgD (IA6-2)

IgD Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

IA6-2

Cat no : PE-65175

Synonyms

FLJ00382, FLJ46727, IgD, IGHD

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Validation Data Gallery

• 

  FC experiment of human peripheral blood lymphocytes using PE-65175

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and 10.00 ul/test PE Anti-Human IgD (PE-65175, Clone:IA6-2). Sample were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-65175

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and PE mouse IgG2a isotype control antibody. Sample were not fixed.

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Tested Applications

• Positive FC detected in: Human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65175 targets IgD in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgD
• Full Name: immunoglobulin heavy constant delta
• Calculated Molecular Weight: 573 aa, 63 kDa
• GenBank Accession Number: BC021276
• Gene Symbol: IGHD
• Gene ID (NCBI): 3495
• RRID: AB_2883949
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.