Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65210-1-Ig targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgG Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
The PIP immunoglobulin is useful as an isotype-matched control. The PIP immunolglobulin is specific for Glutathione S-transferase and can be used as an isotype control for Armenian Hamster IgG antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG Isotype Control antibody 65210-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Armenian Hamster IgG Isotype Control (PIP), catalog number 65210-1-Ig, has 2 indexed citations. These appear in Aging and Disease and The Journal of Immunology. The research fields span neuroimmunology (siponimod therapy in acute intracerebral hemorrhage models) and comparative immunology (B cell heterogeneity in grass carp). Both studies utilized this antibody for flow cytometry applications.
| Species | Application | Title |
|---|---|---|
Aging Dis Lymphocyte-Related Immunomodulatory Therapy with Siponimod (BAF-312) Improves Outcomes in Mice with Acute Intracerebral Hemorrhage | ||
J Immunol An Atlas of Grass Carp IgM+ B Cells in Homeostasis and Bacterial Infection Helps to Reveal the Unique Heterogeneity of B Cells in Early Vertebrates |
