FC experiment of BALB/c mouse splenocytes using PE-65210
1X10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug PE Armenian Hamster IgG Isotype Control (PE-65210, Clone: PIP) (blue) or 0.25 ug PE Anti-Mouse CD3ε (PE-65060, Clone: 145-2C11) (red). Cells were not fixed.
1X10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug PE Armenian Hamster IgG Isotype Control (PE-65210, Clone: PIP) (blue) or 0.25 ug PE Anti-Mouse CD3ε (PE-65060, Clone: 145-2C11) (red). Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage Conditions
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
The PIP immunoglobulin is useful as an isotype-matched control. The PIP immunolglobulin is specific for Glutathione S-transferase and can be used as an isotype control for Armenian Hamster IgG antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
Product Specific Protocols
FC protocol for PE IgG Isotype Control antibody PE-65210
FC experiment of BALB/c mouse splenocytes using PE-65210
1X10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug PE Armenian Hamster IgG Isotype Control (PE-65210, Clone: PIP) (blue) or 0.25 ug PE Anti-Mouse CD3ε (PE-65060, Clone: 145-2C11) (red). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG Isotype Control Monoclonal antibody
Catalog Number
PE-65210
Citations
1
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
