Tested Applications
| Positive FC detected in |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65210 targets IgG Isotype Control in FC, IP applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgG Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883961 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
The PIP immunoglobulin is useful as an isotype-matched control. The PIP immunolglobulin is specific for Glutathione S-transferase and can be used as an isotype control for Armenian Hamster IgG antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE IgG Isotype Control antibody PE-65210 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-65210 is the Armenian Hamster IgG Isotype Control (PIP), a monoclonal antibody used as an isotype-matched negative control. It has 1 total citation. The cited publication appeared in Cell Oncology (2024), studying regulatory factor X1's role in sorafenib-induced ferroptosis in hepatocellular carcinoma via BECN1 transcriptional regulation. The research field encompasses cancer biology, specifically hepatocellular carcinoma and ferroptosis mechanisms.
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Regulatory factor X1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by transcriptional regulation of BECN1 |
