CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MOPC-21

Cat no : CL647-65124

Synonyms

CoraLite®647-conjugated Mouse IgG1 Isotype Control, IgG1, IgG1 Isotype Control, mouse IgG1

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65124

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21) (red), CL647 Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy constant gamma 1 (G1m marker)
• Gene Symbol: Ighg1
• Gene ID (NCBI): 16017
• RRID: AB_2920260
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.