Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

Unconjugated

CloneNo.

MPC-11

Cat no : 65128-1-Ig

Synonyms

IgG2b, IgG2b Isotype Control, Mouse IgG2b Isotype Control

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65128-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2b Isotype Control (65128-1-Ig, Clone:MPC-11) (blue), or Anti-Human CD4 (red) followed by CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained (black). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65128-1-Ig targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy chain 3 (serum IgG2b)
• Gene Symbol: Igh-3
• Gene ID (NCBI): 16016
• RRID: AB_2918419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.