PE-CoraLite® Plus 594 Anti-Human IgM Rabbit Recombinant Antibody

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• PE-CoraLite® Plus 594 Anti-Human IgM Rabbit Recombinant Antibody (PCL594-98744)

IgM Uni-rAb^® Recombinant Antibody for FC

Cat No. PCL594-98744

Clone No.252244H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

human IgM, IGHM, MU, VH

• 

  FC experiment of human PBMCs using PCL594-98744

  1x10^6 human PBMCs were surface stained with APC Anti-Human IgD, and 5 ul PE-CoraLite® Plus 594 Anti-Human IgM Rabbit RecAb (PCL594-98744, Clone: 252244H7) or FcZero-rAb® PE-CoraLite® Plus 594 Rabbit IgG Isotype Control Recombinant Antibody (PCL594-FcA98136, Clone: 240953C9). CD19+ cells were gated. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using PCL594-98744

  1x10^6 human PBMCs were surface stained with 5 ul PE-CoraLite® Plus 594 Anti-Human IgM Rabbit RecAb (PCL594-98744, Clone: 252244H7) (red) or FcZero-rAb® PE-CoraLite® Plus 594 Rabbit IgG Isotype Control Recombinant Antibody (PCL594-FcA98136, Clone: 240953C9) (blue). CD19+ cells were gated. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PCL594-98744 targets IgM in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6742

  Product name: Recombinant Human IgM protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 105-448 aa of BC020240

  Sequence: VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLW

• Full Name: immunoglobulin heavy constant mu
• GenBank Accession Number: BC020240
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• Conjugate: PE-CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IgM (Immunoglobulin M) is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions.