Integrin alpha 4 Monoclonal antibody, PBS Only

• 

  WB analysis using 67040-1-Ig (same clone as 67040-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67040-1-Ig (same clone as 67040-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NK-92 using 67040-1-Ig (same clone as 67040-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 67040-1-Ig (same clone as 67040-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67040-1-PBS in a different storage buffer formulation.

Integrin alpha 4 Monoclonal Antibody for WB, ELISA

Cat No. 67040-1-PBS

Clone No.1B6A3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

ITGA4, 1B6A3, CD49 antigen-like family member D, CD49D, IA4

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Product Information

67040-1-PBS targets Integrin alpha 4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28024

  Product name: Recombinant human Integrin alpha-4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 653-1032 aa of BC156712

  Sequence: SMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD

• Full Name: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Calculated Molecular Weight: 1032 aa, 115 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC156712
• Gene Symbol: Integrin alpha 4
• Gene ID (NCBI): 3676
• RRID: AB_2882354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13612
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.