Integrin alpha 4 Monoclonal antibody

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• Integrin alpha 4 Monoclonal antibody (67040-1-Ig)

Integrin alpha 4 Monoclonal Antibody for WB, ELISA

Cat No. 67040-1-Ig Copied!

Clone No. 1B6A3

Publications (3)

Download datasheet

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

ITGA4, 1B6A3, CD49 antigen-like family member D, CD49D, IA4

• 

  WB analysis using 67040-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67040-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NK-92 using 67040-1-Ig

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 67040-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MOLT-4 cells, Raji cells, U-937 cells, NK-92 cells, THP-1 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67040-1-Ig targets Integrin alpha 4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28024

  Product name: Recombinant human Integrin alpha-4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 653-1032 aa of BC156712

  Sequence: SMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD

• Full Name: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Calculated Molecular Weight: 1032 aa, 115 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC156712
• Gene Symbol: Integrin alpha 4
• Gene ID (NCBI): 3676
• RRID: AB_2882354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13612
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin alpha 4 (ITGA4), also named CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1, and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Publications

What published studies show

Integrin alpha 4 Monoclonal antibody (1B6A3), catalog number 67040-1-Ig, has a total of 3 citations. These appear in Advanced Science, Blood Advances, and iScience. The associated research fields include nanomedicine for acute kidney injury treatment, extracellular vesicle biology and lenalidomide resistance in multiple myeloma, and mesenchymal stem cell-based mRNA intervention for pulmonary fibrosis. All citations used the antibody in Western blot applications.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Adv Sci (Weinh); A pH-Responsive Biomimetic Antioxidant Nanoplatform with Dual Renal Targeting for Synergistic Therapy of Acute Kidney Injury.; Authors - Shichao Zhang
human	WB	Blood Adv; SORT1/LAMP2-mediated Extracellular Vesicle Secretion and Cell Adhesion Are Linked to Lenalidomide Resistance in Multiple Myeloma.; Authors - Tomofumi Yamamoto
human	WB	iScience; Deformability-augmented mesenchymal stem cells post-enucleation for efficient mRNA intervention of pulmonary fibrosis in rodent model.; Authors - Hao Sun