Anti-Human Integrin alpha 4 Rabbit Recombinant Antibody

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• Anti-Human Integrin alpha 4 Rabbit Recombinant Antibody (98794-2-RR)

Integrin alpha 4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98794-2-RR

Clone No.252683F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ITGA4, CD49 antigen-like family member D, CD49D, IA4, Integrin alpha IV

• 

  FC experiment of human PBMCs using 98794-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha 4 Rabbit RecAb (98794-2-RR, Clone: 252683F9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 750 Anti-Human CD14 Rabbit Recombinant Antibody (CL750-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98794-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha 4 Rabbit RecAb (98794-2-RR, Clone: 252683F9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98794-2-RR targets Integrin alpha 4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10494

  Product name: Recombinant human ITA4 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011421 of

  Sequence: \

• Full Name: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Gene Symbol: Integrin alpha 4
• Gene ID (NCBI): 3676
• RRID: AB_3746352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13612
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrin alpha 4 (ITGA4), also named CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1, and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells, ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.