CoraLite® Plus 488-conjugated Integrin alpha-5/CD49e Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-84468-4

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Integrin alpha-5 antibody (CL488-84468-4, Clone: 241685A3 ) at dilution of 1:200.

Integrin alpha-5/CD49e Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84468-4

Clone No.241685A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Integrin Alpha-5, ITGA5, 241685A3, CD49 antigen-like family member E, CD49e

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84468-4 targets Integrin alpha-5/CD49e in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC008786
• Gene Symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08648
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).