FcZero-rAb™ PE Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using PE-FcA98171

  1x10^6 human PBMCs were surface stained with FcZero-rAb™ APC Anti-Human CD3 (UCHT1), and 5 ul PE Anti-Human Integrin alpha-5/CD49e Rabbit RecAb (PE-FcA98171, Clone: 241685D4) or PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

Integrin alpha-5/CD49e FcZero-rAb^TM Recombinant Antibody for FC

Cat No. PE-FcA98171

Clone No.241685D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98171 targets Integrin alpha-5/CD49e in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• GenBank Accession Number: BC008786
• Gene Symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha-5 (ITGA5, also known as CD49e), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).