Integrin Alpha 6 Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin Alpha 6 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 27189-1-AP

Synonyms

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

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Validation Data Gallery

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  WB analysis of A549 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.

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  WB analysis using 27189-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.

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  WB analysis of rat lung using 27189-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 27189-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 27189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of A431 using 27189-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Integrin Alpha 6 (27189-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, mouse skin tissue, rat lung tissue, HepG2 cells
• Positive IHC detected in: human kidney tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Product Information

The immunogen of 27189-1-AP is Integrin Alpha 6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat, bovine, Hamster, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Alpha 6 fusion protein Ag25835
• Full Name: integrin, alpha 6
• Calculated molecular weight: 1073 aa, 119 kDa
• Observed molecular weight: 110-130 kDa
• GenBank accession number: BC136456
• Gene symbol: ITGA6
• Gene ID (NCBI): 3655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
bovine	IF	Cells; Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak; Authors - Xiaolan Zhang
monkey	WB	Virulence; Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.; Authors - Yuchen Li
human	WB	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
Hamster	IF	J Virol; Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.; Authors - Xiu Xin
rat	IF	Vascul Pharmacol; Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6; Authors - Li Wu
human	WB,IHC	J Reprod Immunol; The miR-410-5p /ITGA6 axis participates in the pathogenesis of recurrent abortion by regulating the biological function of trophoblast.; Authors - Shujuan Wu

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C