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Integrin Alpha 6 Polyclonal antibody

Integrin Alpha 6 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 27189-1-AP

Synonyms

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6



Tested Applications

Positive WB detected inA549 cells, A431 cells, mouse skin tissue, rat lung tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27189-1-AP is Integrin Alpha 6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, bovine, Hamster, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Alpha 6 fusion protein Ag25835
Full Name integrin, alpha 6
Calculated molecular weight 1073 aa, 119 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC136456
Gene symbol ITGA6
Gene ID (NCBI) 3655
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
IHC protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineIF

Cells

Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak

Authors - Xiaolan Zhang
monkeyWB

Virulence

Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.

Authors - Yuchen Li
humanWB

Stem Cell Res Ther

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Authors - Ling Leng
HamsterIF

J Virol

Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.

Authors - Xiu Xin
ratIF

Vascul Pharmacol

Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6

Authors - Li Wu
humanWB,IHC

J Reprod Immunol

The miR-410-5p /ITGA6 axis participates in the pathogenesis of recurrent abortion by regulating the biological function of trophoblast.

Authors - Shujuan Wu