Integrin alpha 6 Polyclonal antibody

• 

  WB analysis of A549 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.

• 

  WB analysis using 27189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.

• 

  WB analysis of A431 using 27189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Integrin alpha 6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27189-1-AP

Publications(26)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Synonyms

ITGA6, CD49 antigen-like family member F, CD49f, Integrin alpha-6, Integrin alpha-6 heavy chain

---

Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HepG2 cells, mouse lung tissue, Calu-1 cells, SW480 cells, rat lung tissue
• Positive IHC detected in: mouse skin tissue, human colon tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 10 publications below

Product Information

27189-1-AP targets Integrin alpha 6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin alpha 6 fusion protein Ag25835
• Full Name: integrin, alpha 6
• Calculated Molecular Weight: 1073 aa, 119 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC136456
• Gene Symbol: Integrin alpha 6
• Gene ID (NCBI): 3655
• RRID: AB_2880792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23229
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
bovine	IF	Cells; Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak; Authors - Xiaolan Zhang
monkey	WB	Virulence; Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.; Authors - Yuchen Li
human	WB	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
Hamster	IF	J Virol; Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.; Authors - Xiu Xin
rat	IF	Vascul Pharmacol; Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6; Authors - Li Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Insa (Verified Customer) (03-22-2023)

Did work very well in ICC

• Applications: Other
• Primary Antibody Dilution: 1:100-1:400
• Cell Tissue Type: iPSC derived Astrocytes