Integrin Alpha 6 Polyclonal antibody

Integrin Alpha 6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27189-1-AP

Synonyms

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

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Validation Data Gallery

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  WB analysis using 27189-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, mouse skin tissue, rat lung tissue
• Positive IHC detected in: human kidney tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 27189-1-AP is Integrin Alpha 6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: Hamster, human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Alpha 6 fusion protein Ag25835
• Full Name: integrin, alpha 6
• Calculated molecular weight: 1073 aa, 119 kDa
• Observed molecular weight: 110-130 kDa
• GenBank accession number: BC136456
• Gene symbol: ITGA6
• Gene ID (NCBI): 3655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB,IHC	Neoplasia; NFAT1 Hypermethylation Promotes Epithelial-Mesenchymal Transition and Metastasis in Nasopharyngeal Carcinoma by Activating ITGA6 Transcription.; Authors - Jian Zhang
human	WB,IHC	Am J Transl Res; MiR-302a-5p suppresses cell proliferation and invasion in non-small cell lung carcinoma by targeting ITGA6.; Authors - Wei Chen
human	WB	Biochem Biophys Res Commun; MicroRNA-377-3p alleviates IL-1β-caused chondrocyte apoptosis and cartilage degradation in osteoarthritis in part by downregulating ITGA6.; Authors - Yihui Tu
Hamster	IF	J Virol; Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.; Authors - Xiu Xin
human	WB	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
monkey	WB	Virulence; Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.; Authors - Yuchen Li