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Integrin Alpha 6 Monoclonal antibody

Integrin Alpha 6 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12A1

Cat no : 66906-1-Ig

Synonyms

CD49f, DKFZp686J01244, FLJ18737, Integrin alpha 6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

Validation Data Gallery

Applications:
  • Western Blot (WB) analysis of A431 cells using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    WB analysis of A431 using 66906-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin alpha-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    WB analysis of JAR using 66906-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin Alpha 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    WB analysis of human placenta using 66906-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin Alpha 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    FC experiment of A431 using 66906-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Integrin Alpha 6 (66906-1-Ig, Clone:2B12A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, JAR cells, human placenta tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66906-1-Ig targets Integrin Alpha 6 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Integrin Alpha 6 fusion protein Ag25866
Full Name integrin, alpha 6
Calculated molecular weight 1073 aa, 119 kDa
Observed molecular weight 115-120 kDa
GenBank accession numberBC136456
Gene symbol ITGA6
Gene ID (NCBI) 3655
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha 6 antibody 66906-1-IgDownload protocol
FC protocol for Integrin Alpha 6 antibody 66906-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols