Anti-Human Integrin alpha V Rabbit Recombinant Antibody

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• Anti-Human Integrin alpha V Rabbit Recombinant Antibody (98996-1-RR)

Integrin alpha V Uni-rAb^® Recombinant Antibody for FC

Cat No. 98996-1-RR

Clone No.261824A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ITGAV, CD51, Integrin alpha-V, Integrin alpha-V heavy chain, Integrin alpha-V light chain

• 

  FC experiment of HT-1080 using 98996-1-RR

  1x10^6 HT-1080 cells were surface stained with 0.25 ug Anti-Human Integrin alpha V Rabbit RecAb (98996-1-RR, Clone: 261824A1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 98996-1-RR

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human Integrin alpha V Rabbit RecAb (98996-1-RR, Clone: 261824A1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human peripheral blood platelets using 98996-1-RR

  1x10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human Integrin alpha V Rabbit RecAb (98996-1-RR, Clone: 261824A1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: HT-1080 cells, human peripheral blood platelets, MCF-7 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98996-1-RR targets Integrin alpha V in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Gene Symbol: Integrin alpha V
• Gene ID (NCBI): 3685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06756
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.