Mouse Integrin beta 5 Recombinant monoclonal antibody, PBS Only (Detector)

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• Mouse Integrin beta 5 Recombinant monoclonal antibody, PBS Only (Detector) (87465-1-PBS)

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  WB analysis using 87465-1-RR (same clone as 87465-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87465-1-RR (ITB5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87465-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP03166-1

  The mean Integrin beta 5 concentration was determined to be 88.87 ng/mL in mouse lung extract based on a 5.00 mg/mL extract load, 28.42 ng/mL in mouse heart extract based on a 5.40 mg/mL extract load and 100.97 ng/mL in mouse liver extract based on a 8.40 mg/mL extract load.

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  Sandwich ELISA standard curve of MP03166-1

  Sandwich ELISA standard curve of MP03166-1, Mouse Integrin beta 5 Recombinant Matched Antibody Pair - PBS only. 87465-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg10456. 87465-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  IHC staining of mouse colon using 87465-1-RR (same clone as 87465-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87465-1-RR (Integrin beta 5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87465-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 87465-1-RR (same clone as 87465-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Integrin beta 5 antibody (87465-1-RR, Clone: 252748B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87465-1-PBS in a different storage buffer formulation.

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  Affinity of 87465-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87465-1-RR against Mouse Integrin beta 5 were performed. The affinity constant is 1.11 nM.

Integrin beta 5 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 87465-1-PBS

Clone No.252748B7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

Integrin beta-5, Itgb5

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Product Information

87465-1-PBS targets Integrin beta 5 as part of a matched antibody pair:

MP03166-1: 87465-2-PBS capture and 87465-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10456

  Product name: Recombinant mouse ITB5 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011459 of

  Sequence: \

• Full Name: integrin beta 5
• Observed Molecular Weight: 100 kDa
• Gene Symbol: Itgb5
• Gene ID (NCBI): 16419
• RRID: AB_3745564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrin beta-5 (ITGB5) is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869).