FC experiment of C57BL/6 mouse splenocytes using 65213-1-Ig
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse Integrin beta-7 (65213-1-Ig, Clone: FIB504) or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse Integrin beta-7 (65213-1-Ig, Clone: FIB504) or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65213-1-Ig targets Integrin beta-7 in FC applications and shows reactivity with human, mouse samples.
FC experiment of C57BL/6 mouse splenocytes using 65213-1-Ig
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse Integrin beta-7 (65213-1-Ig, Clone: FIB504) or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Integrin beta-7 Monoclonal antibody
Catalog Number
65213-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
